Strain specific analysis of TAU mRNA expression in wild-type C57BL/6 mice and B-hTAU mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTAU mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TAU primers. Human TAU mRNA was detectable only in homozygous B-hTAU mice but not in wild-type mice. 

Western blot analysis of TAU protein expression in homozygous B-hTAU mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTAU mice (H/H), and then analyzed by western blot with anti-TAU antibody. 40 μg total proteins were loaded for western blotting analysis. TAU was detected in brain from wild-type and homozygous mice.